By J. Szejtli
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Extra info for Cyclodextrin Technology (Topics in Inclusion Science)
Mutagenicity and teratogenicity studies Female rats were fed orally with 200, 400 or 600 mg/kg ~-cyclodextrin from day seven through to day 11 after mating. No signs of toxicity occurred as judged from the conception rate, and the body weight gain. There was no evidence of any effect of the treatment on the number of implantations, the percentage of resorptions, the percentage of dead and live foetuses, the weight of the live foetuses, and the number of foetuses showing major congenital abnormalities [ 314 l.
Since recent toxicological studies have definitely disproved those results [ 340 1. 1) Acute toxicity Defined LD'50 values generally could not be determined, because the administered, rather high, doses resulted in no mortality of the animals. 8 g/kg. and for dogs is more than 5 g/kg. 340 J. The acute LDso value of y-cyclodextrin for mice is more than 16 g/kg, and more than 8 g/kg for rats [ 231 J. ii) Sub-chronic toxicity of ~-cyclodextrin In 90 day and six month oral toxicity studies in rats (Long-Ewans, five weeks old), treated with a daily dose of 200, 400 or 600 mg/kg, the weekly records of body weight did not show any growth depressing effect.
Cramer and Steinle [ 204 J observed that on addition of toluene to the conversion mixture, the yield of tl-cyclodextrin increased continuously. In contrast the yield of a-cyclodextrin, after briefly reaching a maximum, decreased sharply. Without the addition of toluene the main product was a-cyclodextrin accompanied by minor amounts of tl-cyclodextrin. 2% yield of 13- cyclodextrin with only traces of a-cyclodextrin. 1% of tl-cyclodextrin. Under industrial conditions, in the presence of toluene, the conversion of maize starch results in about 49% 13- and 1% a-cyclodextrin.