By Wayne H. Pitcher Jr. (auth.)
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Extra info for Advances in Biochemical Engineering, Volume 10, 1st Edition
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The product of hexokinase action ADP was utilised by polynucleotide diphosphohydrolase to give polyadenylic acid. The rate of synthesis of the polynucleotide by both enzymes, when concentrated in a droplet phase, was markedly higher than in homogeneous Biotechnology of Immobilized Multienzyme Systems 1 Enzyme 2 Enzymes Homogeneous Solution P~ = k'lSbt Attached to an lmpermeable Mem brahe p~ _ AklSbDt (k I 1+ D)v where p l p2 v 1 b A kl, k2 k'l, k~ D 31 k~ Sblk~t + exp ( - k ~ t ) p2b - DklSb Ak2t + vk2 (k I 1+ D) V - ll exp [ -Ak2Dt 1 [v(k 21 + D) 1 v arc product concentrations is solution volume thickness of 'unstixred' layer as subscript indicates bulk solution (x > 1) is surface area in contact with volume v are first order rate constants for enzymes i and 2 are first order rate constants for enzyme I and 2 in solution is the diffusion coefficient Fig.